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Figure 7 | BMC Molecular Biology

Figure 7

From: Regulation of the human LAT gene by the Elf-1 transcription factor

Figure 7

Overexpression of Elf-1 stimulates LAT promoter activity. The pGL3-basic reporter vector containing the -400 to -1 wt region (wt) of the LAT promoter or the -400 to -1 region with point mutations within both Ets sites (mutEts) were transiently transfected into HeLa cells in the presence of the parental pCB6 vector or the expression vector for Elf-1, pCB6-Elf-1. Twenty to twenty four hours later cells were harvested and fold activation was determined as described. The luciferase activity for each construct in the absence of co-transfected Elf-1 was then set at 1-fold. The resulted shown are the average fold induction and s.e.m. for at least three independent experiments. p < 0.05 for Elf-1 activation of the mutant promoter relative to that of wild-type as determined using the two-tailed t test.

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