Figure 7
From: TLK1B promotes repair of UV-damaged DNA through chromatin remodeling by Asf1
A Chromatin assembly (supercoiling assay) of bluescript plasmid with MM3MG extract supplemented with Asf1 and/or TLK1B. Input: Fully supercoiled plasmid prior to incubation in nuclear extract. Plasmid was assembled into chromatin by incubation (for 1 hour at 37°C) with extract, energy mix and the specified components indicated. The figure is a representative of three separate experiments. The densitometric profile for each condition is shown. Note the increase in intensity of band 7 in lane 6. B Chromatin assembly (supercoiling assay) of bluescript plasmid in the presence of TLK1-antiserum (MM3MG extracts). Lane 1: Input plasmid; Lane 2: Plasmid + extract; Lane 3: Plasmid + extract + TLK1-antiserum; Lane 4: Plasmid + extract + pre-immune serum. Lane 5: Plasmid + extract + TLK1-antiserum + recombinant TLK1B re-addition. The assay shown is a representative of two independent experiments. (MM3MG and MM3-TLK1B extracts gave similar results).