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Figure 6 | BMC Molecular Biology

Figure 6

From: Expression and loss of alleles in cultured mouse embryonic fibroblasts and stem cells carrying allelic fluorescent protein genes

Figure 6

Genotyping the ROSA26 locus in DNA isolated from monochromatic clones. At left are shown maps of the two alleles in parental cells. The YFP gene has an additional cleavage site for Pst1 restriction endonuclease. Lane 1, a clone lacking the YFP gene. Lane 2, a dichromatic clone, which retained both the genes, as expected. Lane 3, a clone lacking the CFP gene. Lanes 4 & 5, data from DNA of unsorted ES cell lines. Lane 6, PCR control containing no DNA in the reaction mix. Markers are 1 Kb DNA ladder (Invitrogen).

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