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Table 1 Primers used in the creation of polyadenylation cassettes.

From: Differences in polyadenylation site choice between somatic and male germ cells

Cassette/Template

Primer sequence

CCNA2-SOM1/336M6

5'-GTTCTAGA CTGCTTTGTTTGCCCATTTTTCTC

 

3'-CTTGGATCC CTCCCCACTGTACAATAAAACAA

CCNA2-SOM2/336M6

5'-AATTTCTAGA GGTTAACATTGGTGCTGCCAAC

 

3'-GTTTGGATCC CCCTGAGCAATGAAAGTGGAC

CCNA2-GC1/336M6

5'-GTCTAGA GTAGGAAAAGATTTTATAAAAC

 

3'-CATGGATCC CTGTAACATCTGAGAGCCGATC

CCNA2-GC2/336M6

5'-CTCTAGA GAATCTTACCCTGTTCTAAGTAAG

 

3'-CGGATCC CATGGGCAGTCCTGGTAGCAAG

CREM-somatic/304K8

5'-TCTAGA GTCACGTAGGTCTAGACTGGC

 

3'-GGATCC CTGAGTTCGGATCCCAACACC

CREM-MGC/304K8

5'-TCTAGA AACAAGAAGCTTCTAGAGGAGC

 

3'-GGATCC CCTTAGAGGTAGGATCCAATTAAATAC

c-abl-somatic/156H9

5'-TCTAGA CCAGTACATTTTGTTTCTAGATATGACTG

 

3'-GGATCC CTGCTGTGAGGATCCCAAGAG

c-abl-mgc/156H9

5'-TCTAGA TTCCCTGTCCTTCTAGAAC

 

3'-GGATCC GATGGACCTTCTAGACACCAAG

  1. Shown at left are the names of the polyadenylation cassettes with the BAC template used as a template to create each cassette, and at right the sequences of the primers used to make the respective polyadenylation cassette. The XbaI and BamHI restriction enzyme sites used for cloning are shown in bold and italics, respectively.
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BMC Molecular Biology

ISSN: 1471-2199

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