Selection of reference genes for quantitative RT-PCR studies in striped dolphin (Stenella coeruleoalba) skin biopsies

Table 2 Details of primers and amplicons for each of the 10 evaluated genes.

Gene	Forward Primer Sequence [5'→3']	Position in cDNA	Reverse Primer Sequence [5'→3']	Position in cDNA	Amplicon Length	E%	R²
Act-B	CCATCGGCAATGAGCGGTTCC	3^rd Exon	CGTGTTGGCATACAGGTCCTTCC	4^th Exon	146bp	97.9	0.997
B2M	TGGCTCCTTTCGTGACCTTGG	1^st Exon	GTCGTGAGTAAACCTGAACCTTCG	2^nd Exon	96bp	99	0.996
GAPDH	CAAGGCTGTGGGCAAGGTCATC	7^th Exon	TTCTCCAGGCGGCAGGTCAG	7^th Exon	111bp	97.4	0.995
HPRT1	GTCAAGCAGCATAATCCAAAGATG	6^th Exon	GTCTGGTCTATATCCAACACTTCG	7^th Exon	84bp	98.1	0.998
PGK1	ACAATGGAGCCAAGTCAG	3^rd Exon	CACGCAGTCCTTCAAGAAC	4^th Exon	146bp	97.4	0.996
RPL4	CAGACCTTAGCAGAATCTTGAAAAGC	8^th Exon	CCTGGCGAAGAATGGTGTTCC	9^th Exon	171bp	99.9	0.998
RPS18	CAATTAAGGGTGTGGGGCGAAG	2^nd/3^rd Exon	TCTTGTATTGGCGTGGATTCTGC	4^th Exon	141bp	100.8	0.999
SDHA	TCTTTCCCACCAGGTCACACAC	3^rd Exon	CCAGTCGGAGCCCTTCACG	4^th Exon	119bp	88.2	0.993
TFRC	ACCATCTCAGTCATCAGGATTG	8^th Exon	CATTCTTGTTCTGTGAGGATACC	9^th Exon	152bp	98.1	0.998
YWHAZ	AAATGAAAGGAGACTACTACCGCTA	2^nd Exon	AGACCCAATCTGATAGGATGTGTTG	3^rd Exon	151bp	95.9	0.994

Primers are located in different exons, except for GAPDH, where primers are located in the same exon. E is a measure of real-time reaction efficiency and R² indicates reproducibility of the reaction.

ISSN: 1471-2199