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Table 1 Transformation efficiency of the E. coli lysogens carrying stx2-phages

From: Use of the lambda Red recombinase system to produce recombinant prophages carrying antibiotic resistance genes

 

pBC-SK+

pKD46

 

Vector +

stx+

Vector +

stx+

DH5α (VTB55)

99.0a

100.0

100

6

DH5α (312)

98.5

96.5

100

1

DH5α (534)

100.0

99.5

100

80

DH5α (549)

97.5

100.0

100

5

DH5α (557)

100.0

100.0

100

3

C600(A9)

99.2

100.0

100

100

C600(933W)

100.0

100.0

100

100

  1. aPercentage of colonies positive for the presence of each vector performed in parallel in two plates, relative to the total number of colonies grown in plates with the respective antibiotic. Results were obtained by colony hybridization of the colonies present in a plate.