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Figure 2 | BMC Molecular Biology

Figure 2

From: Use of the lambda Red recombinase system to produce recombinant prophages carrying antibiotic resistance genes

Figure 2

A) Number of transductants (CFU/ml) obtained in E. coli DH5α and E. coli C600 with each recombinant phage carrying ca t or tet antibiotic resistance genes. B) PCR products of each recombinant colony carrying a stx-prophage with the respective antibiotic resistance cassette. 1: stx gene control, 2: cat control, 3: tet control, 4–5: E. coli DH5α and E. coli C600 lysogens with stx-phages::cat. 6–7: E. coli DH5α and E. coli C600 lysogens with stx-phages::tet-. 8 negative PCR control; C) As an example, colony blot of DH5α (Ø557::tet) hybridized with the specific probe for the tet gene.

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BMC Molecular Biology

ISSN: 1471-2199

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