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Figure 2 | BMC Molecular Biology

Figure 2

From: The transcription factor ATF3 is upregulated during chondrocyte differentiation and represses cyclin D1 and A gene transcription

Figure 2

Atf3 expression is upregulated during chondrocyte differentiation in vivo. Tibia isolated from embryonic day 15.5. mice were microdissected into the resting/proliferating, prehypertrophic and hypertrophic areas, and RNA was isolated directly out of cartilage. Expression of type II collagen II (Col2a1), type X collagen (Col10a1) and Atf3 was examined by real-time PCR analyses and normalized to Gapdh expression. All data represent averages and SEM from four independent experiments (*: p < 0.05). Col2a1 was strongly expressed in the resting/proliferating area and declined subsequently (A). Col10a1 was virtually undetectable in the resting/proliferating area and strongly induced in the prehypertrophic and hypertrophic areas (B). Atf3 was already expressed in the resting/proliferating area, but expression was significantly increased in the more mature zones of the growth plate.

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