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Table 2 Evaluation of ABCC1 Fold Change Using Different Housekeeping Genesa

From: Plasma membrane calcium ATPase (PMCA4): A housekeeper for RT-PCR relative quantification of polytopic membrane proteins

Gene Used for Relative Quantification

Fold Change of ABCC1 in COR-L23R cells Compared to parental COR-L23P cells

PMCA4

90.7 ± 7.1

GAPDH

407.8 ± 98.9

HPRT1

152.3 ± 58.9

  1. aThe delta-delta Ct method was used with the various reference genes to calculate the fold change in ABCC1 for the COR-L23R cells compared to the parental COR-L23P cells.