Plasma membrane calcium ATPase (PMCA4): A housekeeper for RT-PCR relative quantification of polytopic membrane proteins

Table 1 Comparison of Housekeeping Gene Expression in Various Cell Lines^a

Cell line	Housekeeping Genes
	ATP1A1 ^b	GAPDH	HPRT1	PMCA4
Human Salivary Gland cells	19.62 ± 0.38	16.96 ± 0.11	21.33 ± 0.21	20.61 ± 0.14
HCT-116 (colorectal carcinoma)	20.4 ± 0.67	17.33 ± 0.4	21.71 ± 0.31	21.7 ± 0.36
KB-3-1 (Cervical squamous cell carcinoma)	20.57 ± 0.36	17.73 ± 0.07	20.49 ± 0.57	22.13 ± 0.1
SNB-75 (Glioblastoma)	19.77 ± 0.27	17.49 ± 0.15	21.9 ± 0.2	19.77 ± 0.17
SF-295 (Glioblastoma)	20.62 ± 0.34	17.01 ± 0.43	21.48 ± 0.28	21.61 ± 0.13
MCF-7 (breast adenocarcinoma)	18.49 ± 0.28	17.1 ± 0.33	20.69 ± 0.13	21.39 ± 0.24
UACC-62 (melanoma)	18.66 ± 0.8	16.31 ± 0.5	21.22 ± 0.25	20.58 ± 0.3
COR-L23P (lung carcinoma)	18.14 ± 0.34	18.12 ± 0.24	20.54 ± 0.21	21.03 ± 0.17

^aThe LightCycler RNA Master SYBR Green Kit and LightCycler 480 were used in these RT-PCR studies. The average crossing point values are given for the different cell lines ± SD (n = 4 for all samples).
^bF-test comparing all values for PMCA4 and ATP1A1 indicates that there is a statistically significant difference (p = 0.041). No statistically significant difference is seen between PMCA4 and GAPDH (p = 0.093) or PMCA4 and HPRT1 (p = 0.074)

ISSN: 1471-2199