Figure 1From: Plasma membrane calcium ATPase (PMCA4): A housekeeper for RT-PCR relative quantification of polytopic membrane proteinsStandard curve to evaluate efficiency of RT-PCR reaction. The HPRT1 (blue circle) and PMCA4 (red diamond) primers were evaluated over a range of total RNA concentrations (30 ng-300 ng total RNA). The average crossing point values are given for the n = 4, and the error bars represent ± SD.Back to article page