Characterization of novel alternative splicing sites in human telomerase reverse transcriptase (hTERT): analysis of expression and mutual correlation in mRNA isoforms from normal and tumour tissues

Table 1 Primer pairs used for hTERT PCR analysis

Target ¹⁾	Primer pair ²⁾	Ampl. (bp)
intron 2–15	p1255: ggaattctggagctgcttgggaacca m3652: cgtctagagccggacactcagccttca	2409
intron 2	p1252: tgtttctggagctgcttgggaacca m1725: tcatcagccagtgcaggaacttg	474
intron 2–3	p1509: tggggctccaggcacaacgaa m1955: cttggggatgaagcggagtct	447
intron 4	p1870: tgcgggagctgtcggaagcagag m2170: cgcacacgcagcacgaaggt	301
intron 5–9	p2182: cgccgcctgagctgtactttgtc m2681: gctctagatccaccaaacgcaggagca	507
intron 10–13	p2660: gctgctcctgcgtttggtggatgat m3164: ggggttcttccaaacttgctgatg	505
intron 14–15	p3194: ggcctccctctgctactccatcct m3652: cgtctagagccggacactcagccttca	466
intron 14–15	p3194: ggcctccctctgctactccatcct m3925: gggcacacctttggtcactcc	732

¹⁾ Positions of indicated introns are included in PCR product. ²⁾ Primer sequences are from 5' to 3'. p, forward primer; m, reverse primer.

ISSN: 1471-2199