Table 4 Primer and MGB Taqman probe sequences and concentrations used in real time PCR.
Gene symbol | Primer/probe | Gene Sequence (5'-3') | Concentration (nM) |
|---|---|---|---|
ACT1 | Forward | TTTCATCTTCTGTATCAGAGGAACTTATTT | 300 |
Reverse | ATGGGATGAATCATCAAACAAGAG | 300 | |
MGB probe | ATTATTTTCGTCACGGGTATT | 300 | |
RPP2B | Forward | TGCTTACTTATTGTTAGTTCAAGGTGGTA | 300 |
Reverse | CAACACCAACGGATTCCAATAAA | 300 | |
MGB probe | CACCTCTCCATCAGCTT | 200 | |
TDH1 (GAPDH) | Forward | CGGTCCATCCCACAAGGA | 300 |
Reverse | AGTGGAAGATGGGATAATGTTACCA | 300 | |
MGB probe | AGGTGGTAGAACTGC | 200 | |
PMA1 | Forward | TTGCTTATGATAATGCTCCATACGA | 300 |
Reverse | TACCCCACAATCTTGGCAAGT | 300 | |
MGB probe | CCAAAACCAGTTAAATGG | 200 | |
LSC2 | Forward | CGTCAACATCTTTGGTGGTATTGT | 900 |
Reverse | TTGGTGGCAGCAATTAAACCT | 900 | |
MGB probe | AGATGTGATTACGTCGCCA | 400 | |
CPA1 | Forward | TCTGGTGTTGCTGCCATAACTG | 300 |
Reverse | AATTCTCCCCAATGATGAACCTT | 300 | |
MGB probe | AGACAATTGGTGAGTTATT | 200 | |
IMH 3 | Forward | TATTCATATGGCATTATTGGGTGGTA | 600 |
Reverse | AACCATTTCTGCTTGTTCTTCAGA | 600 | |
MGB probe | TGGTATCATTCATCATAACTGTAC | 400 | |
RIP | Forward | TGTCACGGTTCCCATTATGATATTT | 300 |
Reverse | TGGAATTTCCAAGTTCAATGGA | 300 | |
MGB probe | TGGTAGAATTAGAAAGGGTCCA | 200 | |
ALS1 | Forward | CAACAGGCACCTCAGCATCTAC | 300 |
Reverse | CTCCACCAGTAACAGATCCACTAGTAA | 300 | |
MGB probe | AGTGCTAATAGCGAACTT | 300 | |
ALS3 | Forward | CAACTTGGGTTATTGAAACAAAAACA | 300 |
Reverse | AGAAACAGAAACCCAAGAACAACCT | 300 | |
MGB probe | AAACTAGCTGTGAAGGTGAT | 300 |