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Table 3 The mean expression ratios of the ALS1 and ALS3 genes in all the C. albicans biofilm samples compared to all the planktonic samples calculated with four different normalization strategies.

From: Development and evaluation of different normalization strategies for gene expression studies in Candida albicans biofilms by real-time PCR

Genes Fold expression calculated with four different normalization strategies
ALS1 5HK 3HK ACT1 cDNA
Mean 2.98 3.67 5.12 3.35
SD 1.64 2.11 2.33 1.53
Range 0.67–7.54 0.68–8.41 1.85–10.58 1.08–6.57
ALS3 5HK 3HK ACT1 cDNA
Mean 0.069 0.083 0.15 0.10
SD 0.02 0.022 0.069 0.033
Range 0.042–0.13 0.044–0.14 0.072–0.35 0.046–0.16