AtMND1 is required for homologous pairing during meiosis in Arabidopsis

BMC Molecular Biology

Table 2 Female gametophytic defects in the Atmnd1 mutant.

Ovule Stages	1n		2n		4n		8n		MES		Degen
	m	+	m	+	m	+	m	+	m	+	m	+
3-1	31	100	ND	ND	ND	ND	ND	ND	ND	ND	69	ND
3-2	25	60	9	40	ND	ND	ND	ND	ND	ND	66	ND
3-4	39	2	4	40	7	52	ND	5	ND	ND	50	0.8
3-6	32	ND	6	2	0.4	7	0.2	7	0.6	83	60	1.2

Stages of female gametophyte development relative to ovule stages in the Atmnd1 mutant and wild type. A total of 759 and 637 ovules were scored for the Atmnd1 mutant and wild-type respectively. Megagametogenesis defects are evident from stage 3-1 in case of the mutant, which leads to embryo sac arrest and eventually its degeneration. Ovule stages are according to [54]: 3-1, 3-2, and 3-4 are postmeiotic stages of the sporophyte with respect to elongation of the integuments where the wild type gametophyte would normally reach the 1n, 2n, and 4n stages; 3-6 corresponds to a mature ovule prior to fertilization. MES, Mature embryo sac; Degen, Degenerated embryo sac; M, mutant; +, wild type; ND, Not detected.

ISSN: 1471-2199

Ovule Stages	1n		2n		4n		8n		MES		Degen
	m	+	m	+	m	+	m	+	m	+	m	+
3-1	31	100	ND	ND	ND	ND	ND	ND	ND	ND	69	ND
3-2	25	60	9	40	ND	ND	ND	ND	ND	ND	66	ND
3-4	39	2	4	40	7	52	ND	5	ND	ND	50	0.8
3-6	32	ND	6	2	0.4	7	0.2	7	0.6	83	60	1.2

Ovule Stages	1n		2n		4n		8n		MES		Degen
	m	+	m	+	m	+	m	+	m	+	m	+
3-1	31	100	ND	ND	ND	ND	ND	ND	ND	ND	69	ND
3-2	25	60	9	40	ND	ND	ND	ND	ND	ND	66	ND
3-4	39	2	4	40	7	52	ND	5	ND	ND	50	0.8
3-6	32	ND	6	2	0.4	7	0.2	7	0.6	83	60	1.2

Ovule Stages	1n		2n		4n		8n		MES		Degen
	m	+	m	+	m	+	m	+	m	+	m	+
3-1	31	100	ND	ND	ND	ND	ND	ND	ND	ND	69	ND
3-2	25	60	9	40	ND	ND	ND	ND	ND	ND	66	ND
3-4	39	2	4	40	7	52	ND	5	ND	ND	50	0.8
3-6	32	ND	6	2	0.4	7	0.2	7	0.6	83	60	1.2