Figure 2

Construction of CDR1 promoter-Renilla luciferase. The PCR products containing the promoter and the translation initiation site of CDR1 (open box) were introduced in-frame with the sequence encoding luciferase (RLUC, hatched box) into the plasmid pGEM with the wild-type URA3 gene as the selective marker. The LOB 85 plasmid was linearized with Eco RV, transformed, and integrated into the chromosome at the promoter of CDR1 (solid box) in C. albicans.