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Table 1 Transcript variants for ACSL1 and ACSL6 identified in various tissues. Variants for ACSL1 and 6 were identified from cDNAs by PCR amplification of the Gate-domain region (see Methods). The number of clones representing each of the variants found in the population of sequenced clones is given as a ratio and is shown under each respective variant.

From: Multiple erythroid isoforms of human long-chain acyl-CoA synthetases are produced by switch of the fatty acid gate domains

 

ACSL1 1

ACSL6 2

CD34 + cells

v2 – v3 (34/35 – 1/35)

v1 – v2 (19/22 – 3/22)

Reticulocytes

not detected

v1 – v2 – v4 – v5 (3/8 – 4/8 – 1/8) – (2/4)3

K-562 cells

v1 – v2 – v3 (1/11)3– (29/30 – 1/30)

v1 – v2 – v4 (24/26 – 1/26 – 1/26)

  1. 1ACSL1_v1 was found in placenta (1/10). ACSL1_v2 was found in fetal blood liver (11/11) and placenta (9/10).
  2. 2ACSL6_v3 could not be found by PCR screening of an additional 38 clones.
  3. 3Clones identified by RACE-PCR in an independent experiment.