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Figure 3 | BMC Molecular Biology

Figure 3

From: Multiple erythroid isoforms of human long-chain acyl-CoA synthetases are produced by switch of the fatty acid gate domains

Figure 3

Detection of ACSL6 isoforms in E. coli (A) and in human K562 cells (B). A: vector (1, 2, 3); ACSL6_v2 (4, 5, 6); ACSL6_v3 (7, 8, 9). Crude extract (1, 4, 7); Soluble fraction (2, 5, 8); Membrane fraction (3, 6, 9). Detection performed with Pierce INDIA HIS-antibody. Protein ladder standard is shown on the right (top to bottom: 250-150-100-75 kDa). Proteins carried a hexahistidine-tag at their amino-terminus (see Methods). B: Total protein extract of K562 cells. Detection performed with a peptide raised antibody against ACSL6.

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