Two splice forms of mouse Tgif2. A) Expression of mouse Tgif2 was analyzed by PCR from 1st strand cDNA, using primers at the 5' and 3' ends of the coding sequence. cDNAs were from the tissues indicated (sk. musc = skeletal muscle), or from embryos at the indicated days of gestation. -ve: no cDNA. Similar PCR analysis was carried out on cDNA from human brain and kidney (right). B) Alignment of putative splice sequences from mouse and human TGIF2 with splice consensus sequences (matches to the consensus are shaded gray).