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Table 1 DNAzyme and LNAzyme sequences and their RNA substrates. The catalytic sequence (loop) is invariable in all the nucleic acid enzyme constructions and has the sequence 5'-GGCTAGCTACAACGA. RNA modifications in the hybridization arms are indicated by bold underlined nucleotides; the cytidine LNA residue in Lz6-1096 is methylated at the 5-position of the base. The RNA substrates that were cleaved by the respective enzymes are underlined, those that could not be cleaved are italicized (the enzyme:substrate ratios for cleavage are defined in Fig. 3).

From: Locked nucleoside analogues expand the potential of DNAzymes to cleave structured RNA targets

Enzyme code

Sequence (5' to 3')

Type of modification

RNA substrates (cleaved uncleaved)

Dz4-745

TTTTTCAA loop ATTAGTCG

none

74n, 2904n

Lz7-745

TTT TT CAA loop ATT AGT CG

α-L-LNA

74n, 2904n

Lz8-745

TTT TT CAA loop ATT AGT CG

LNA

74n, 2904n

Dz1-1093

AGCTATTA loop GCTTTCTT

none

17n, 33n, 58n, 2904n

Lz1-1093

AGCT ATT A loop GCT TT CTT

α-L-LNA

17n, 58n, 2904n

Lz2-1093

AGCT ATT A loop GCT TT CTT

LNA

17n, 58n, 2904n

Lz5-1093

AGCT ATT A loop GCT TT CT T

α-L-LNA

58n, 2904n

Dz2-1093

TATTA loop GCTTTC

none

17n, 2904n

Lz4-1093

T ATT A loop GCT TT C

α-L-LNA

17n, 58n, 2904n

Lz3-1093

ATT A loop GCTT

α-L-LNA

17n, 58n, 2904n

Dz3-1096

GTGAGCTA loop TACGCTTT

none

33n, 58n

Lz6-1096

GTGA GCT A loop TAC GCT TT

LNA

33n, 58n