Figure 4

Kinetics of cleavage at the 1093-site by LNAzymes. (A) Single turnover cleavage of 17n by Dz1-1093 (circles), Lz1-1093 (squares) and Lz2-1093 (diamonds). The enzymes are in 5 × excess. (B) Single turnover cleavage of 58n by Dz1-1093 (in 50 × excess), Lz2-1093 (in 5 × excess), and Lz2-1093 (in 5 × excess). (C) Multiple turnover cleavage of 17n by Lz1-1093 and Lz2-1093. (D) Lineweaver-Burk plot showing Lz1-1093 data points obtained over a full range of substrate concentrations (5 nM to 1.2 μM), and used for determination of K_m and V_max.