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Figure 2 | BMC Molecular Biology

Figure 2

From: Locked nucleoside analogues expand the potential of DNAzymes to cleave structured RNA targets

Figure 2

RNA substrates for LNA- and DNAzyme cleavage. RNA substrates for LNA- and DNAzyme cleavage. (A) 17n minimal RNA substrate; a 33n RNA with metastable secondary structure; and 58n and 74n RNAs that fold into well-defined secondary structures. Cleavage sites for the nucleic acid enzymes at nucleotides 745, 1093 and 1096 are shown by the arrows. The stem-loop secondary structures containing the cleavage targets have been verified by numerous techniques including biochemical probing [28], NMR [29] and crystallography [30-32]. (B) Secondary structure of domain II of 23S RNA (2904n RNA), which contains all three cleavage sites. (C) Higher-order folding of the rRNA (2904n) within the 50S ribosomal subunit; all the stem-loops in the smaller RNAs used here are maintained in the 2904n RNA (refs, PDB 1JJ2, [33]).

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