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Table 1 Relative luciferase activity of the DRD4 promoter constructs. Luciferase activities of the crude cell extracts were normalized to their β-galactosidase activities. Data are shown as the mean ± SD. Results of a representative experiment are shown as measured in triplicates. Similar data were obtained from three independent transfection experiments.

From: No direct effect of the -521 C/T polymorphism in the human dopamine D4 receptor gene promoter on transcriptional activity

Luciferase/β-gal ratio SK-N-F1 Y79 IMR32 HeLa
pGL3-Basic 3.52 ± 1.43 3.87 ± 0.09 3.70 ± 0.29 3.59 ± 0.13
pDRD4-A 10.35 ± 1.00 8.80 ± 1.29 6.77 ± 0.86 8.71 ± 0.78
pDRD4-B 31.63 ± 4.7 26.89 ± 4.51 10.56 ± 0.52 27.08 ± 1.54
pDRD4-C 34.09 ± 3.34 28.98 ± 4.61 9.45 ± 0.46 21.89 ± 0.99
pDRD4-D 0.42 ± 0.02 1.75 ± 0.15 1.97 ± 0.12 2.56 ± 0.14
pDRD4-E 1.66 ± 0.20 4.18 ± 0.38 1.76 ± 0.22 2.51 ± 0.31
pDRD4-F 1.75 ± 0.17 4.21 ± 0.23 1.77 ± 0.16 2.63 ± 0.30
pTK-luc 84.21 ± 10.76 114.97 ± 10.50 28.73 ± 2.29 172.35 ± 2.05