Figure 8
Expression of human podocalyxin in HEK293 and Meg0l cells treated with 5-AzaC. Cells were treated with 5-AzaC for 48 hrs. Cell lysates were resolved on SDS-7.5% polyacrylamide gels under non-reducing conditions, transferred to PVDF membranes and immunoblotted with A) 1 μg/ml of anti-podocalyxin mAb and B) 1 μg/ml of anti-Sp1 mAb. β-Actin was blotted as a loading control. The experiment was repeated three times.