Figure 7

State of methylation of CpG dinucleotides in the human podocalyxin promoter. A) Schematic representation of a fragment of the Podxl-pr comprising 208 nts upstream the transcription start site (indicated with an arrow) and the 5'UTR. Grey boxes denote the positions of CpG dinucleotides. Underlined nts indicate Sp1 recognition sites. B) Genomic DNA from Tera-1 or Meg0l cells were extracted and subjected to bisulfite treatment, PCR amplification and DNA sequencing as described in Methods. CpG sites are numbered from 1 to 27 relative to the transcription start site. Three bisulfite treatments were performed. Data is expressed as % of methylation, calculated as percentage of conserved C in at least 10 clones obtained after each bisulfite treatment.