Figure 5

Identification of Sp1-DNA complexes by supershift assays. A) Different probes were designed as described under "Methods" in order to identify functional Sp1 sites in the Podxl promoter. B) Antibodies (2 μg) for Sp1 and non-specific, were preincubated with 5 μg of Meg01 nuclear extracts (NE) before the addition of the wild or mutated Sp1-containing Podxl probes. BSA (2 μg) was used as a control. An arrow points to the supershifted Sp1 bands.