Figure 4

Gel mobility-shift analysis of DNA-protein complexes using the human podocalyxin promoter fragment -243/-18. A) Western blots were performed as described under Fig. 2; B) Five μg of nuclear extracts (NE) from Tera-1 and Meg0l cells were incubated with the probe. Competitive gel shift assay was done in the presence of 100-fold molar excess of unlabeled double-stranded oligonucleotides corresponding to the pointed out transcription factors consensus and unrelated nonspecific (Non Sp) motifs. Antibodies (2 μg) for AP2 or non-specific were preincubated with the Tera-1 and Meg0l NE before the addition of the Podxl probe.