Figure 3

Effect of Sp1 on podocalyxin gene promoter activity in insect cells. Drosophila-derived SL2 cells were transiently cotransfected with 5 μg of pGL3/Podxl-pr constructs and with either 0.5 μg of pPAC void plasmid or 0.5 μg of pPAC-Sp1 plasmid. Luciferase activities were normalized by protein concentration and are shown as the fold increases relative to the basal activity of void pGL3-basic plasmid (control). Values reported in the figure are mean ± SEM of three independent experiments in duplicates.