Figure 1

Transient transfection of deletion mutants of the 5'-flanking region of the human podocalyxin gene. Description of the construction of the pGL3-Podxl-pr reporter plasmids and transient transfection experiments are described under "Methods". Luciferase activity was corrected for transfection efficiency with the values obtained with Renilla and was expressed as percent of the maximal activity of the -364 construct. The results are mean ± SEM of three experiments performed in duplicates.