Table 1 List of oligonucleotides used in this study
From: Flexible promoter architecture requirements for coactivator recruitment
Plasmid construction | |
|---|---|
Oligo name | Sequence (5'→ 3') [restriction sites underlined] |
HIS3_F_BamHI | CGGGATCC CGAAGATGACAGAGCAGAAAGC |
HIS3_R | CCTCGTTCAGAATGACACG |
MEL1_NcoI_W | CCCTGAAAGGTTTTTCCATGG AATAGTCAGGACGC |
MEL1_NcoI_C | GCGTCCTGACTATTCCATGG AAAAACCTTTCAGGG |
ATG_W | GTAATAAAAGCAACGACGTTGAACGGATCCCGAAAG |
ATG_C | CTTTCGGGATCCGTTCAACGTCGTTGCTTTTATTAC |
pMH14-F_ApaI | ATAGGGCCC GGAAATTTGTGTAAAACCCCC |
pMH14-R_AscI-SacII | AACAACCGCGG ATAATGGCGCGCC CCTCGTTCAGAATGACACG |
pUG73_F | AAGGCGCGCC GCATAGGCCACTAGTGGATCTG |
pUG73_R | AGTAAGGCGCGCC CAGCTGAAGCTTCGTACGC |
Promoter library construction | Â |
Oligo name | Sequence (5'→ 3') [restriction sites underlined] |
MET16_reverse | CCGCTCGAGTTACTGAAGTTG |
Cbf1_6_Met31 | CATGCCATGG TATCATCATTTCACGTGGCCACAACTTCAGTAACTCGAG CGG |
Cbf1_8_Met31 | CATGCCATGG TATCATCATTTCACGTGGNNCCACAACTTCAGTAACTCGAG CGG |
Cbf1_10_Met31 | CATGCCATGG TATCATCATTTCACGTGGNNNNCCACAACTTCAGTAACTCGAG CGG |
Cbf1_12_Met31 | CATGCCATGG TATCATCATTTCACGTGGNNNNNNCCACAACTTCAGTAACTCGAG CGG |
Cbf1_14_Met31 | CATGCCATGG TATCATCATTTCACGTGGNNNNNNNNCCACAACTTCAGTAACTCGAG CGG |
Cbf1_16_Met31 | CATGCCATGG TATCATCATTTCACGTGGNNNNNNNNNNCCACAACTTCAGTAACTCGAG CGG |
Cbf1_18_Met31 | CATGCCATGG TATCATCATTTCACGTGGNNNNNNNNNNNNCCACAACTTCAGTAACTCGAG CGG |
Cbf1_20_Met31 | CATGCCATGG TATCATCATTTCACGTGGNNNNNNNNNNNNNNCCACAACTTCAGTAACTCGAG CGG |
Cbf1_22_Met31 | CATGCCATGG TATCATCATTTCACGTGGNNNNNNNNNNNNNNNNCCACAACTTCAGTAACTCGAG CGG |
Cbf1_24_Met31 | CATGCCATGG TATCATCATTTCACGTGGNNNNNNNNNNNNNNNNNNCCACAACTTCAGTAACTCGAG CGG |
Cbf1_26_Met31 | CATGCCATGG TATCATCATTTCACGTGGNNNNNNNNNNNNNNNNNNNNCCACAACTTCAGTAACTCGAG CGG |
Cbf1_28_Met31 | CATGCCATGG TATCATCATTTCACGTGGNNNNNNNNNNNNNNNNNNNNNNCCACAACTTCAGTAACTCGAG CGG |
Cbf1_30_Met31 | CATGCCATGG TATCATCATTTCACGTGGNNNNNNNNNNNNNNNNNNNNNNNNCCACAACTTCAGTAACTCGAG CGG |
Cbf1_32_Met31 | CATGCCATGG TATCATCATTTCACGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNCCACAACTTCAGTAACTCGAG CGG |
Cbf1_34_Met31 | CATGCCATGG TATCATCATTTCACGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCACAACTTCAGTAACTCGAG CGG |