Figure 3
From: Flexible promoter architecture requirements for coactivator recruitment
Synthetic promoter system. A minimal promoter from the MEL1 gene (P MEL1 ) was fused upstream of a HIS3 reporter gene on a single-copy plasmid. Selected restriction enzyme sites are labeled with their coordinates. Various combinations of Cbf1 and Met31/32 binding sites were inserted between the NcoI and XhoI restriction enzymes sites in the minimal promoter.