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Table 1 Primers and oligonucleotides used in C/EBP mutagenesis. Primers and oligonucleotides

From: Functional characterisation of the regulation of CAAT enhancer binding protein alpha by GSK-3 phosphorylation of Threonines 222/226

Constructs DNA sequences
Flag-C/EBPα Forward: 5'-GGA TCC GCC ACC ATG GAC TAG AAG GAC GAC GAT GAC AAG GAG TCG GCC GAC TTC TAC GAG-3'
Reverse: 5'- GAA TTC TCA CGC GCA GTT GCC CAT GGC CTT GAC-3'
S230A Flag-C/EBPα mutant Sense: 5'-CGC CCG TGC CCG CCC CTC ATC CCG-3'
Antisense: 5'-CGG GAT GAG GGG CGG GCA CGG GCG-3'
Flag-C/EBPα mutant(AA) Sense:5'-GGC CAC CCT GCG CCG CCG CCG GCG CCC GTG CCC AGC CCT CAT CCC-3'
Antisense: 5'-GGG ATG AGG GCT GGG CAC GGG CGC CGG CGG CGG CGC AGG GTG GCC-3'
GSK3α-V5 Forward: 5'-GGA TCC GCC ACC ATG AGC GGC GGC GGG CCT T-3'
Reverse: 5'-TCT AGA GGA AGA GTT AGT GAG GGT AGG TGT GGCA-3'
CBE-Luc reporter Sense:5'-CGG GGT ACC CCG ATT TTT GCG CAA TTT TAT TGC GCA ATC AAT ATT GGA AGA TCT TC- 3'
Antisense: 5'-GAA GAT CTT CCA ATA TTG ATT GCG CAA TAA AAT TGC GCA AAA ATC GGG GTA CCC CG-3'