Figure 7

C/EBPα activity in H4IIE cells. (A) H4IIE cells were transfected with the CBE-Luc (10 μg) along with 1 μg of pcDNA6, Flag-C/EBPα, Flag-C/EBPα-AA, Flag-C/EBPα-AAA or Flag-C/EBPα-S230A prior to a 20 h incubation with insulin (10 nM) or CHIR99021 (CT, 2 μM). Results are presented as relative luciferase activity to control (serum free) and are average ± SEM (n = 6) from two independent experiments. * p = 0.0683, ** p = 0.0523. (B) H4IIE cells were co-transfected with 10 μg of either CBE-Luc reporter or pGL3-Luc reporter plus 1 μg of pcDNA6 or Flag-C/EBPα prior to 20 h incubation with dexamethasone (500 nM), insulin (10 nM), or CHIR99021 (CT, 2 μM). Results are presented as relative luciferase activity to the control (serum free) and are average ± SEM (n = 6) from two independent experiments. ***p < 0.001