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Figure 5 | BMC Molecular Biology

Figure 5

From: A potential role of alternative splicing in the regulation of the transcriptional activity of human GLI2 in gonadal tissues

Figure 5

Tissue-specificity of GLI2 mRNA and its alternatively spliced forms. Southern blot analysis of the PCR products amplified from cDNAs derived from (A) normalized multiple tissue panels and (B) different cell lines (indicated on top of the panel) using GLI2-specific primers derived from exons 2 and 7. The products were hybridized with a 918 nt-riboprobe encompassing GLI2 exons 2–7. Bars on the right indicate sizes of PCR products corresponding to GLI2 mRNA and its alternatively spliced forms GLI2 Δ3 and GLI2 Δ4–5 (0.9, 0.8 and 0.5 kb, respectively). A shorter and longer exposure were used to determine the presence of minor transcripts corresponding to GLI2 Δ3 (0.8 kb) in ovary and testis (panel A). Note the complex pattern of alternatively spliced products observed for cell lines (panel B) that is most likely due to promiscuous splicing (not analyzed in detail). Control, no cDNA.

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