Table 2 Binding site mutants. Positions of mutations in mutant constructs. Schematic illustration of the P2X1 core promoter mutation constructs. "." indicates positions same as wild type. "~" indicates where sequence has been omitted for clarity.
From: Sp1/3 and NF-1 mediate basal transcription of the human P2X1 gene in megakaryoblastic MEG-01 cells
|  | NF-κB | NF-1 | Sp1a | Sp1b |
|---|---|---|---|---|
Wild type | GGGGAGTCCCC~~~TGGTTTCCTGCCAAC~~~GGGGCTGGG~~~CCCCGCCCC | |||
mNFkB | ..CC.......~~~...............~~~.........~~~......... | |||
mNF-1 | ...........~~~.........TAA...~~~.........~~~......... | |||
mSp1a | ...........~~~...............~~~..TT.....~~~......... | |||
mSp1b | ...........~~~...............~~~.........~~~.....AA.. | |||
mut1 | ..CC.......~~~.........TAA...~~~.........~~~......... | |||
mut2 | ...........~~~...............~~~..TT.....~~~.....AA.. | |||
mut3 | ...........~~~.........TAA...~~~.........~~~.....AA.. | |||
mut4 | ...........~~~.........TAA...~~~..TT.....~~~.....AA.. | |||