Table 1 Oligonucleotides used for EMSA. Primer sequences are 5' to 3'. Bold lowercase bases represent mutations with respect to the wild type sequence. Underlined sequences represent consensus binding sites for respective transcription factor.
From: Sp1/3 and NF-1 mediate basal transcription of the human P2X1 gene in megakaryoblastic MEG-01 cells
Name | Position | Sequence |
|---|---|---|
O-NFκB | -168/-144 | GAGTCGCGGGGAGTCCCC TAATCTG |
O-NFκB-mut |  | GAGTCGCGGcc AGTCCCCTAATCTG |
O-NF1 | -29/-5 | CCTGTGG TTTCCTGCCAAC AACTCC |
O-NF1-mut | Â | CCTGTGGTTTCCTtaa AACAACTCC |
O-Sp1a | +33/+60 | GGAGGGGCAGTGGGGCTGGG CCTGGGTG |
O-Sp1a-mut | Â | GGAGGGGCAGTGGtt CTGGGCCTGGGTG |
O-Sp1b | +63/+90 | CCCACCAGCCCCGCCCC ATCTATCTTTG |
O-Sp1b-mut | Â | CCCACCAGCCCCGaa CCATCTATCTTTG |