Comparing protocols for preparation of DNA-free total yeast RNA suitable for RT-PCR

BMC Molecular Biology

Table 1 Oligonucleotides used as primers in RT-PCR analysis

Target mRNA	Primer Sequence	Amplicon Size (bp)
*TPS1*	Forward CGCTAAGGCGCAACTGACCTCGTCT Reverse CGACGAGAATGCGTGGTTGGCATAC	379
*TPS2*	Forward CCCCCCAAACTATCAGATTGGAACAAC Reverse ACCCAGCTGCAGCTATTCCATCGGC	612
*PDA1*	Forward GGTCAGGAGGCCATTGCTGT Reverse GACCAGCAATTGGATCGTTCTTGG	673
*CNA1*	Forward CGAAAGACTTGAATTCTTCACGCATC Reverse GAATGATCTGCAGCAAGCATCG	629
*CNA2*	Forward CCTTATATCTGTTCCCGCCC Reverse GAGGAACCATGGTTTTGGAG	551
*ACT1*	Forward CCTACGTTGGTGATGAAGCT Reverse GTCAGTCAAATCTCTACCGG	407

CNA1, CNA2, PDA1 and ACT1 primer sequences were obtained from Del Aguila et al, 2003 and TPS1 and TPS2, from Souza, 2001.

ISSN: 1471-2199