Figure 3

Effect of C-terminal and N-terminal deletion mutants of the E1A protein on the activity of the K18 promoter. (A) Schematic representation of the structure of the E1A-13S protein and of the deletion mutants. Know domains of E1A-13S (CR1, CR2, CR3 and CR4 and the N-terminal region) are shown as boxes. Numbers indicate positions in aa. (B and C) SW613-3 (T) or SW613-B3 (NT) cells were transfected with plasmid pK18(80)luc alone (none) or in the presence of the indicated E1A constructs. Luciferase activities are expressed relative to that obtained with the pK18(80)luc plasmid alone. Error bars represent the standard deviation of the mean of at least two experiments.