Table 1 RAMP-1 5' RACE analysis. Total RNA was extracted from mouse brain, skeletal muscle and heart. RNA was treated with alkaline phosphatase, tobacco acid pyrophosphatase and ligated to a small adapter. Following reverse transcription, multiple rounds of PCR were performed and the PCR products cloned and sequenced. Transcriptional initiation sites are indicated relative to the start site of translation (ATG = 0).
Mouse RAMP-1 transcriptional start sites | |||
|---|---|---|---|
Clone | Brain | Muscle | Heart |
1 | -41 | -50 | -41 |
2 | -41 | -50 | -41 |
3 | -41 | -40 | -41 |
4 | -9 | -40 | -9 |
5 | -9 | -50 | -35 |
6 | -11 | -35 | -35 |
7 | -50 | -9 | -9 |
8 | -41 | -11 | -50 |
9 | -50 | - | -41 |
10 | -35 | - | - |