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Figure 2 | BMC Molecular Biology

Figure 2

From: Cloning and transcriptional analysis of the mouse receptor activity modifying protein-1 gene promoter

Figure 2

Semi-quantitative RT-PCR on RAMP-1 gene expression in C2C12 cells. Total RNA was isolated from C2C12 non-confluent cells (nc), confluent (c) and myotubes (mt). The RNA was subjected to reverse transcription followed by PCR with mouse RAMP-1 and actin specific primers. The PCR reactions were stopped after 30, 35, 40 cycles and size fractionated on a 1% agarose gel. The DNA was then Southern blotted overnight and hybridised with a 32P end-labelled RAMP-1 and actin specific probes followed by autoradiography. This experiment is representative of three experiments each giving similar results.

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