Recognition and binding of mismatch repair proteins at an oncogenic hot spot

BMC Molecular Biology

Table 1 Mismatch repair at an oncogenic site within MMR proficient and MMR deficient human nuclear extracts.

	HCT 116 + Ch. 3; H-ras codon 12 middle G:C		HCT 116; H-ras codon 12 middle G:C
	G:T	G:A	G:T	G:A
Total repair ^a (correct & incorrect)*	18.2 %	30 %	∅^b	∅
Correct repair ^a (% of total)**	(96%)	(60%)	∅	∅
Incorrect repair ^a (% of total)**	(4%)	(40%)	∅	∅
Total # analyzed	99	100	78	91

^a Repair efficiency above background for each mismatch at H-ras codon 12 by each human nuclear extract was determined by the following equations [39].
Total repair efficiency= 100 × (1 - [fraction of nonrepaired mixture incubated with human nuclear extract results / fraction of nonrepaired results of direct transformation of NR9161]).
Correct repair efficiency= 100 × {(fraction of correctly repaired incubated with human nuclear extract) - [(fraction of correct repair by NR9161 direct transformation) × (1 - fraction of total repair efficiency)]}.
Incorrect repair efficiency= Total repair efficiency - Correct repair efficiency.
^b No repair detected above background.
* Designates statistical difference between HCT116 + Ch.3 G:T and G:A repair at P < 0.05.
**Designates statistical difference between HCT116 + Ch.3 G:T and G:A repair at P < 0.005.

ISSN: 1471-2199