Figure 7
From: Heterologous expression in Tritrichomonas foetus of functional Trichomonas vaginalis AP65 adhesin
T. foetus transfected with pBS- ap65-neo (Tf-pBS- ap65-neo ) displays enhanced levels of adherence to immortalized human MS-74 VECs compared to T. foetus (Tf) parasites. The percent level of adherence was adjusted with that seen for T. vaginalis (Tv). T. foetus with control plasmid gave levels of adherence similar to T. foetus (data not shown). The enhanced adherence obtained with transfected T. foetus (Tf-pBS-ap65-neo) was inhibited by anti-AP65 IgG (hatched middle bar). The solid gray bar (right) represents inhibition by normal rabbit serum (NRS) control. The results are the average from four different experiments, and each experiment was carried out using quadruplicate samples. The statistical significance of the results is indicated by the asterisk above the bar graph.