Skip to main content
Figure 3 | BMC Molecular Biology

Figure 3

From: Heterologous expression in Tritrichomonas foetus of functional Trichomonas vaginalis AP65 adhesin

Figure 3

Monoclonal antibody (mAb) detects AP65 expressed in pBS- ap65-neo (Parts A and B) and pBS- ap65-HA-neo (Part C) transfected T. foetus parasites. (A) Total proteins from 107 parasites were separated on 10% SDS-PAGE and blotted onto Hybond-P membranes for probing with mAb DM116 specific for AP65, as before (Garcia et al., 2003). The AP65 protein was readily detected in total protein blots of transfected T. foetus (lane 2) and T. vaginalis (lane 4), but not in wt T. foetus (lane 1) and in T. foetus transfected with the control pBS-neo plasmid (lane 3). The migration of detected AP65 was ~65-kDa as expected based on molecular weight standards. (B) A ligand assay showing AP65 binds to immortalized human MS-74 VECs, as before (Garcia et al., 2003). Parasites bound to VECs were solubilized and electrophoresed as above for blotting onto Hybond-P membranes. The DM116 mAb was used to detect AP65 in transfected T. foetus (lane 2) and T. vaginalis (lane 4). No protein was detected in wt T. foetus (lane 1) and T. foetus transfected with the control plasmid (lane 3).

Back to article page