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Figure 1 | BMC Molecular Biology

Figure 1

From: Heterologous expression in Tritrichomonas foetus of functional Trichomonas vaginalis AP65 adhesin

Figure 1

Tritrichomonas foetus has lower levels of adherence to HeLa epithelial cells and immortalized human MS-74 VECs and has no detectable ap65 adhesin gene. (A) Bar graph showing the mean level of adherence by T. foetus (shaded bars) derived from four independent adherence experiments, each of which was performed with quadruplicate samples. Adherence levels of T. vaginalis (open bars) were normalized to 100% for comparative purposes. (B) Northern analysis to detect ap65 mRNA amounts was performed using 10 μg of total RNA per lane separated on 1.2% agarose-formaldehyde gels and transferred to Hybond-P membranes. The blot was probed with DIG-labelled ap65, which hybridized to an ~2-kb ap65 transcript in T. vaginalis, as before [16]. No similar signal was detected in T. foetus and the drug resistant MR100 T. vaginalis used as a negative control [15]. (C) Ethidium bromide (EtBr)-stained RNA gel showing the rRNA bands. Equal loading of RNA in all the lanes is evident by similar amounts of the rRNA bands, which served as internal controls for the Northern blot in part B.

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