KLF4 R390S mutant has a novel BglII restriction site. a) pCS2-KLF4-R390S construct diagram. b) ClaI / BglII Restriction digest of both wild-type and successfully mutated plasmid DNA. c) α-Flag Western blot showing expression of mutant construct in 293T cells. d) Sequencing result of the mutation, mutated residue is highlighted in red.