Table 2 Standard curve evaluation of potential reference genes in Atlantic salmon.
From: Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmon
Thymus | Muscle | Intestine | Head kidney | |||||
|---|---|---|---|---|---|---|---|---|
Slope | R2 | Slope | R2 | Slope | R2 | Slope | R2 | |
18S rRNA | -3.26 | 0.996 | -3.40 | 0.993 | -3.23 | 0.992 | -3.27 | 0.998 |
S20 | -3.28 | 0.996 | -3.21 | 0.991 | -3.29 | 0.990 | -3.07 | 0.993 |
β-actin | -2.73 | 0.994 | -3.36 | 0.997 | -3.11 | 0.997 | -3.05 | 0.996 |
GAPDH | -3.38 | 0.997 | -3.21 | 0.995 | -3.37 | 0.996 | -3.13 | 0.996 |
EF1AA | -3.06 | 0.996 | -3.05 | 0.993 | -3.05 | 0.994 | -3.06 | 0.996 |
EF1AB | -3.11 | 0.998 | -3.13 | 0.998 | -3.20 | 0.993 | -3.31 | 0.997 |
Gills | Brain | Liver | Spleen | |||||
Slope | R2 | Slope | R2 | Slope | R2 | Slope | R2 | |
18S rRNA | -3.41 | 0.999 | -3.32 | 0.992 | -3.42 | 0.998 | -3.20 | 0.995 |
S20 | -3.29 | 0.990 | -3.22 | 0.991 | -3.39 | 0.991 | -3.28 | 0.995 |
β-actin | -3.10 | 0.999 | -3.21 | 0.997 | -3.23 | 0.990 | -3.44 | 0.994 |
GAPDH | -3.57 | 0.997 | -3.48 | 0.902 | -3.21 | 0.995 | -2.83 | 0.714 |
EF1AA | -3.38 | 0.998 | -2.86 | 0.994 | -3.11 | 0.994 | -3.15 | 0.992 |
EF1AB | -3.32 | 0.991 | -3.14 | 0.991 | -3.24 | 0.991 | -3.52 | 0.996 |