Skip to main content

Table 2 Standard curve evaluation of potential reference genes in Atlantic salmon.

From: Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmon

  Thymus Muscle Intestine Head kidney
  Slope R2 Slope R2 Slope R2 Slope R2
18S rRNA -3.26 0.996 -3.40 0.993 -3.23 0.992 -3.27 0.998
S20 -3.28 0.996 -3.21 0.991 -3.29 0.990 -3.07 0.993
β-actin -2.73 0.994 -3.36 0.997 -3.11 0.997 -3.05 0.996
GAPDH -3.38 0.997 -3.21 0.995 -3.37 0.996 -3.13 0.996
EF1AA -3.06 0.996 -3.05 0.993 -3.05 0.994 -3.06 0.996
EF1AB -3.11 0.998 -3.13 0.998 -3.20 0.993 -3.31 0.997
  Gills Brain Liver Spleen
  Slope R2 Slope R2 Slope R2 Slope R2
18S rRNA -3.41 0.999 -3.32 0.992 -3.42 0.998 -3.20 0.995
S20 -3.29 0.990 -3.22 0.991 -3.39 0.991 -3.28 0.995
β-actin -3.10 0.999 -3.21 0.997 -3.23 0.990 -3.44 0.994
GAPDH -3.57 0.997 -3.48 0.902 -3.21 0.995 -2.83 0.714
EF1AA -3.38 0.998 -2.86 0.994 -3.11 0.994 -3.15 0.992
EF1AB -3.32 0.991 -3.14 0.991 -3.24 0.991 -3.52 0.996