Table 1 TaqMan assays used to evaluate potential reference genes in Atlantic salmon.
From: Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmon
Gene | Forward primer | Reverse primer | TaqMan MGB probe | Amplicon size (bp) |
|---|---|---|---|---|
18S rRNA | CCCCGTAATTGGAATGAGTACACTTT | ACGCTATTGGAGCTGGAATTACC | CACCAGACTTGCCCTCC | 98 |
S20 | GCAGACCTTATCCGTGGAGCTA | TGGTGATGCGCAGAGTCTTG | CCTCAAGGTGAAGGGA | 85 |
β-actin | CCAAAGCCAACAGGGAGAAG | AGGGACAACACTGCCTGGAT | TGACCCAGATCATGTTT | 91 |
GAPDH | AAGTGAAGCAGGAGGGTGGAA | CAGCCTCACCCCATTTGATG | CTGATCATTGGAAACCT | 96 |
EF1AA | CCCCTCCAGGACGTTTACAAA | CACACGGCCCACAGGTACA | ATCGGTGGTATTGGAAC | 57 |
EF1AB | TGCCCCTCCAGGATGTCTAC | CACGGCCCACAGGTACTG | CCAATACCGCCGATTTT | 59 |