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Figure 6 | BMC Molecular Biology

Figure 6

From: cAMP response element binding protein (CREB) activates transcription via two distinct genetic elements of the human glucose-6-phosphatase gene

Figure 6

Biological activity of constitutively active ATF2 fusion protein towards glucose-6-phosphatase promoter-containing reporter genes. (A) Schematic representation of the modular structure of C2/ATF2. This chimeric bZIP protein consists of the constitutively active transcriptional activation domain of CREB2 and the bZIP domain of ATF2, responsible for dimerization and DNA-binding. (B, C, D) HepG2 cells were transfected with one of the reporter plasmids pG6PCRE1/CRE2luc, pG6PCRE1mut/CRE2luc pG6PCRE1/CRE2mutluc (B), pG6PCRE1luc, pG6PCRE2luc (C), or pTNFα(CRE/AP1)2luc (D), the pRSVβ internal reference plasmid, and either the "empty" expression vector pCMV5 or an expression vector encoding C2/ATF2 (100 ng expression plasmid/plate). Lysates were prepared forty-eight hours post-transfection and β-galactosidase and luciferase activities were measured. The mean +/- SD is depicted.

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BMC Molecular Biology

ISSN: 1471-2199

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