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Figure 1 | BMC Molecular Biology

Figure 1

From: cAMP response element binding protein (CREB) activates transcription via two distinct genetic elements of the human glucose-6-phosphatase gene

Figure 1

The human glucose-6-phosphatase gene promoter. (A) Sequence of a portion of the human glucose-6-phosphatase gene promoter including the CRE-like sequences CRE1 and CRE2. (B) The reporter plasmid pG6PCRE1/CRE2luc contains a minimal promoter consisting of the human immunodeficiency virus TATA box, the adenovirus major late promoter initiator element, the luciferase open reading frame, and glucose-6-phosphatase promoter sequences encompassing both CRE-like sequences CRE1 and CRE2. The reporter plasmids pG6PCRE1mut/CRE2luc and pG6PCRE1/CRE2mutluc carry mutations in either the CRE1 or the CRE2, to inactivate these sites. (C) Reporter plasmids pG6PCRE1luc and pG6PCRE2luc contain glucose-6-phosphatase promoter sequences encompassing either the CRE1 or the CRE2. (D) The reporter plasmid pTNFα(CRE/AP1)2luc contains two identical copies of the composite CRE/AP1 sequence derived from the human TNFα gene, upstream of a minimal promoter. The sequence of one of these motifs is depicted.

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BMC Molecular Biology

ISSN: 1471-2199

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