Phosphorylation of H3 and H2AX after irradiation. MM3MG and MM3MG over-expressing TLK1B cells were grown to 80% confluence prior to gamma-radiation (10 Gy). Cells were harvested at different times (0 h, 30 min, 1 h, 2 h, 4 h, 8 h and 16 h) after irradiation and lysed in RIPA buffer. Equal amount of protein of each sample was loaded on a 15% SDS-PAGE gel and electrophoresed. Blots were probed with phospho-Histone H3 (Ser-10) antibody, or phospho-Histone H2AX (Ser-139) antibody (Upstate Cell Signaling). Equal loading of proteins was confirmed by staining the blots with Ponceau S prior to processing. These blots are representative of two separate experiments.